10倍基因组推出两种新产品解锁广泛的单细胞分析铬样品
10倍基因组推出两种新产品解锁广泛的单细胞分析铬样品
The Fixed RNA Profiling Kit enables researchers to fix their tissue at the time of collection, store it, and proceed to a single cell workflow at a time and place of their choosing. The kit, which combines entirely new chemistry and chip with a formaldehyde fixation to preserve the biological state at the point of collection, enables batching of samples for maximum study flexibility. In addition, the kit provides improved sensitivity and more efficient sequencing as well as built-in sample multiplexing capabilities. With the ability to probe cell surface protein expression for multiomic studies, the Fixed RNA Profiling Kit is compatible with any human single cell suspension.
"Single cell measurements have been transformative to our understanding of human health and disease, but the research potential has been hampered by cost," said Peter Skene, Ph.D., Director of Molecular Biology & Biochemistry at the Allen Institute for Immunology. "New multiplexed technologies promise to significantly reduce the cost and boost global research. By locking the cell state, fixed cell measurements will allow unprecedented insight into drug mechanisms, including intracellular protein measurements. Taken together, the new era of single cell research will be a paradigm shift phase in translational research."
Nuclei Isolation Kit
For researchers with unsequenced archival tissue, 10x Genomics developed the Nuclei Isolation Kit to unlock frozen tissues for single cell analysis, answering a major customer request. Standard methods for nuclei isolation from frozen tissue include complex, low-throughput and time-consuming protocols, expensive instruments for sorting and debris removal, and the need to optimize workflows for each tissue. The Nuclei Isolation Kit is a simple, scalable product designed to remove those barriers. With an hour of time and a benchtop centrifuge, the kit enables the generation of high quality nuclei suspensions from a wide range of frozen samples on Chromium, without any expensive instruments or complicated pipetting.
The Fixed RNA Profiling Kit is available now for pre-order, while the Nuclei Isolation Kit is expected to open for pre-order soon. Both kits are expected to begin shipping in mid-May.
In addition, earlier this month, 10x Genomics began shipping both 5' CRISPR, an extension to Single Cell Immune Profiling to make large scale, functional genomics studies quicker and easier; and ATAC v2, an update to the Single Cell ATAC-seq workflow to boost sensitivity while increasing discovery power and reducing costs.
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10倍基因组学、生命科学领袖重点掌握生物学推进人类健康,宣布两个新产品的商业可用性旨在提高样品制备工作流和扩大公司采用领先的单细胞分析:铬平台固定RNA分析及核隔离板。在一起,这些装备将使容易单细胞multiomic脆弱的人类组织样本的分析,使冷冻样本分析,目前昂贵和费时,更容易。
“单细胞分析是生物医学研究和健康的未来带来变革和医学,”Ben Hindson说创始人兼首席科学官10倍基因组学。“我们相信,我们今天宣布的新功能将使未来前进,帮助实现单细胞分析的扩展潜力更大的规模,通过移除障碍多站点研究和支持更广泛的采用单细胞在转化研究方法。”
Hindson继续说道,“这些新的套件是最早的几个新产品计划在我们的铬,今年Visium和宾客礼物平台。我们努力使2022年最令人兴奋的产品发布在我们的历史。”
固定的RNA分析工具
目前,单细胞研究人员必须精心计划和协调他们的实验,以维持细胞生存的生活样本,迅速从样本采集到处理工作。
“RNA序列的关键限制因素是资源的可用性的集合,可以在正常工作时间以外由于各种原因,”卢西亚诺Martelotto说,博士,副教授,单细胞阿德莱德大学的实验室和空间技术。“固定RNA分析工具包允许固定细胞的单个细胞悬液后立即准备,使存储未来实验的细胞不依赖低温贮藏,可以扭曲原样品的细胞成分。”
固定RNA分析工具使研究人员去修复他们的组织的收集,储存,并进入一个细胞工作流在他们选择的时间和地点。装备,全新的化学和芯片相结合,甲醛固定保护生物学状态的集合,支持批处理的样本研究最大的灵活性。此外,该工具包提供了改进的敏感性和更高效的排序以及内置的样品多路复用功能。能够探测细胞表面蛋白表达multiomic研究固定RNA分析设备兼容任何人类单个细胞悬液。
“单一细胞测量已经改变我们对人类健康和疾病的理解,但是研究已经阻碍了潜在的成本,”Peter弧拱说博士,分子生物学和生物化学主任艾伦研究所免疫学。“新的多路复用技术承诺显著减少成本并提高全球研究。通过锁定细胞状态,固定细胞测量将允许前所未有的洞察力药物机制,包括细胞内蛋白质测量。综上所述,单细胞研究的新时代将在转化研究范式转变的阶段。”
核隔离设备
为研究者unsequenced档案组织,10倍基因组学开发核隔离工具对解冻组织单细胞分析,回答一个主要的客户要求。核标准方法隔绝冰冻组织包括复杂、低吞吐量和耗时的协议,昂贵的仪器进行排序和碎片移除,需要为每个组织优化工作流程。细胞核隔离工具是一个简单的、可伸缩的产品旨在消除这些障碍。一个小时的时间和台式离心机,装备使一代的高质量的原子核从各种冷冻样品悬浮液铬,没有任何昂贵的仪器或复杂的移液。
固定RNA分析工具包可以现在预订,而原子核隔离设备预计将很快开放接受预订。这两个包预计在5月中旬开始发货。
此外,本月早些时候,10倍基因组学开始航运两个5 ' CRISPR,扩展单一细胞免疫分析大规模,功能基因组学研究方便、快捷;和ATAC v2更新单细胞ATAC-seq工作流来提高灵敏度,同时增加发现电力和降低成本。
