New Mass Spectrometry Methods To Visualize Lipids

在细胞水平上更深入地看到自然基础的新科学方法的发展导致了上个世纪的一些最大进步。现在，对许多生物学和病理过程所必需的膜脂质家族的新研究，这是涉及人类健康和疾病的功能多功能膜脂质家族之一，在质谱法中使用了进一步的发展。继续推迟导致许多疾病的新疗法的障碍。

In the past, due to various reasons related to their complexity and their low intracellular concentrations, the profiling of these lipids and the linking of a specific acyl variant to biological change has been difficult. However, a new system called PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography and Mass Spectrometry) has now enabled the characterization of the dynamics of phosphoinositide acyl variants both in intracellular and extracellular environments.

先前测量和分析磷酸肌醇的方法已经产生了无法轻易应用于实验动物的临床或病理样本的结果。甚至涉及使用质谱法的新方法，在某些领域的进步仍然反映了如何同时量化生物样品中各个区域异构体的酰基变体的问题。

The PRMC-MS method now solves this problem and points the way to an understanding of how these lipids influence cell functions. Using PRMC-MS, it is now possible to simultaneously measure all eight classes of phosphoinositides in a single sample. The highly sensitive nature of PRMC-MS allows for the detection of tiny but important changes in intracellular phosphoinositide levels, yielding data that shows that it can be applied to blood samples to track phosphoinositide signatures potentially related to disease states.

PRMC-MS enables the comprehensive analysis of phosphoinositide acyl variants in various types of biological samples, including surgical specimens, which can be used to throw a light on previously unrecognized disturbances of phosphoinositide fatty acyl profiles in cancerous tissue and to monitor their extracellular mobilization. Further study of the differing acyl variants and their conferring of protein binding properties could possibly also reveal how they activate a signaling pathway that favors cancer cell growth and survival and emerge as a target for cancer therapy. Thus, PRMC-MS may well illuminate the role played by phosphoinositides in the pathogenesis of cancers and inflammatory diseases.

In addition, the use of PRMC-MS in the evaluation of phosphoinositide signatures at the acyl variant level in tissue and liquid biopsies may reveal biomarkers suitable for a wide variety of clinical applications.

将来，诸如上面的应用可能会极大地促进基于制定治疗剂的药物开发策略，该治疗剂可以确定特定的致病性磷酸肌醇酰基变体，从而为更准确的治疗方法和治疗患者打开了道路。过去证明困难的疾病范围。

Reference:Morioka S，Nakanishi H，Yamamoto T等。一种质谱法，用于对磷酸肌醇区域异构体的深入分析及其疾病相关的调节。纳特社区。2022; 13（1）：83。doi：10.1038/s41467-021-27648-z

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