基本成纤维细胞生长因子（BFGF，也称为FGF2）在体外广泛用于维持和刺激各种细胞。然而，通过BFGF在生理温度下迅速降解的事实，使用天然BFGF在细胞生物学中的使用受到限制。72小时后，我们证明了天然BFGF的活性<20％。我们已经用BFGF的工程形式解决了这个问题，称为Heat稳定BFGF（HS BFGF），该形式在37摄氏度下稳定。HS BFGF在标准的哺乳动物培养条件下72小时后与天然蛋白质保持> 90％的同源性和> 80％的活性。我们已经使用HS BFGF来增强多能人类和大鼠神经干细胞（NSC）的扩展，分别降低了BFGF浓度和更易于用户友好的工作流程。通过使用HS BFGF与天然BFGF的使用，这两种模型的两倍时间也减少了约30％。至关重要的是，在HS BFGF和天然BFGF之间，NSC分化为神经元，星形胶质细胞和少突胶质细胞分离是可比的。下游的分化表明，需要在需要时完全从培养中去除HS BFGF的能力。还测试了HS BFGF在三维（3D）培养物中的MCF7人乳腺癌细胞刺激中进行了测试。 MCF7 spheroids were cultured in bFGF supplemented serum-free medium for 8 days without media changes so that spheroid morphology was undisturbed. Spheroids cultured in HS bFGF were perceptibly larger than those in native bFGF, starting just three days after seeding and continuing throughout the culture period. HS bFGF enhanced spheroid growth by almost 20% more than the native protein, as measured by PrestoBlue® assay. Because there was no need to change the media, we were also able to observe morphological changes to the spheroids that likely would have been impossible to document with a standard media change regimen. These data reinforce the idea that bFGF is a mitotic agent for MCF7 cells, a point which has previously been debated in the literature, and confirm that HS bFGF can provide stable stimulation in long-term culture. Taken together, these data show HS bFGF has broad applicability in cell biology and will be a valuable technology for a variety of applications moving forward. HS bFGF not only allows for reduced concentrations of bFGF to be used, but also enables a more user-friendly workflow for cell culture maintenance.